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. 2022 Oct 19;127(12):2207–2219. doi: 10.1038/s41416-022-02011-1

Fig. 3. In vitro activity of mTOR siRNA-loaded neutral liposome.

Fig. 3

a In vitro mTOR inhibitory activity of NL-C-siRNA (50 nmol/L) and NL-mTOR-siRNA (25 nmol/L) using western blot analysis. b Pictorial representation and c graphical representation of MTT assay for cell viability analysis following treatment of MCF-7 cells with NL-C-siRNA (50 nmol/L) and NL-mTOR-siRNA (10, 25 and 50 nmol/L). d, f Inhibitory effects of NL-C-siRNA and NL-mTOR-siRNA on the invasion of MCF-7 cells as represented by photomicrographs and graph showing in vitro invasion and measured using transwells coated with Matrigel. e, g Representative photomicrographs of wounded monolayers of MCF-7 cells taken at 24 h to determine the effect of NL-C-siRNA and NL-mTOR-siRNA on the migration of MCF-7 breast cancer cells. The migration was estimated by measuring the cells within the wounded region after 24 h. h Cell adhesion rate of MCF-7 cells at 30, 60 and 90 min after treatment with NL-C-siRNA and NL-mTOR-siRNA respectively. The data are represented as mean ± SEM, n = 3 and significance of difference is indicated as ***p < 0.001 and nsp > 0.05. (NL-C-siRNA control siRNA-loaded neutral liposome, NL-mTOR-siRNA mTOR-siRNA-loaded neutral liposome, MTT [3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide]).