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. 2022 Oct 19;127(12):2207–2219. doi: 10.1038/s41416-022-02011-1

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Fig. 7 — a Representative TUNEL fluorescent images of section of tumour excised from rats of individual groups. Blue fluorescence (Hoechst: dark blue) indicates cell nuclei location and the purple fluorescence (Alexa Flour 594: purple) denotes apoptotic cell location. The scale bars are represented as 100 µm. b Estimation of rate of tumour apoptosis in rats belonging to each group in accordance to images of TUNEL assay in which each value shows mean ± SEM (n = 6). Induced control: SD/DMBA+/+, cancerous animals subjected to treatment with NL-C-siRNA: SD/DMBA+/+ NL-C-siRNA, cancerous animals subjected to treatment with NL-mTOR-siRNA: SD/DMBA+/+ NL-mTOR-siRNA. ***p < 0.001, **p < 0.01, *p < 0.05 and ^nsp > 0.05. (NL-C-siRNA control siRNA-loaded neutral liposome, NL-mTOR-siRNA mTOR-siRNA-loaded neutral liposome, DMBA 7,12-dimethylbenz[a]anthracene, SD Sprague Dawley).