Fig. 3. Pore-lining backbone amides affect fast and slow gating in CLC-0.

A Normalized G–V curves for fast gate of CLC-0 WT (black), A417α (orange), F418φ (cyan) and V419ω (pink) in Cl⁻. Solid lines are fits to Eq. 2. Values are mean ± St. Dev. of n > 7 repeats from N ≥ 3 independent oocyte batches. B ΔV_1/2= (V_1/2^mut − V_1/2^WT) of the normalized fast gate G–V of WT and mutant CLC-0 in Cl⁻, Br⁻, NO₃⁻ and I⁻. Colors as in A. Errors represent the propagation of the uncertainty of the V_1/2 parameter evaluated from the fits of the data in A  and Supplementary Fig. 7. C) Representative slow gate current traces of WT and mutant CLC-0. Scale bars indicate 0.5 µA and 1 s. D Normalized G–V curves for slow gate of CLC-0 WT (black), A417α (red), F418φ (green) and V419ω (yellow) in Cl⁻. Solid lines are fits to Eq. 2. Values are mean ± St. Dev. of n > 7 repeats from N ≥ 3 independent oocyte batches. Raw data for A–D  is included in the Source Data Files.