Fig. 4. A glutamate side chain at the Gluex position modulates the role of Scen and Sext in anion selectivity.
A, B Effect of replacing backbone amides with ester oxygens lining the Sext and Scen sites in V166E bCLC-k (A) and E166A CLC-0 (B) on PBr (black bars), PNO3 (red bars) and PI (green bars). Data are Mean ± St.Dev. of n > 4 repeats from N ≥ 3 independent oocyte batches, individual data points are shown as cyan circles. The statistical significance of the effects of the mutants on the permeability ratios of each ion (indicated by *) was evaluated with a one-sided Student’s t-test with a Bonferroni correction (see “Methods”). Mean values and p values are reported in Supplementary Table 1. Individual data points are shown grouped by ion in Supplementary Fig. 5. Raw data for A, B is included in the Source Data Files.