Table 1.
Chicory sample #3 treated with individual enzymes or mixtures (5 mg/g protein) were assessed for total reducing sugars (DNS) and antimicrobial activity against S. aureus
| Enzyme treatment | Release of hydrolysis products as reducing sugarsa | Antimicrobial activity against S. aureus E-70045 | |
|---|---|---|---|
| (mg/mL) | (% of dry matter) | ||
| Inulinase | 6.5 | 13.0 | + |
| Pectinase | 20.1 | 40.1 | + + |
| Cellulase | 6.9 | 13.8 | + |
| β-glucosidase | 4.0 | 8.0 | + |
| Xylanase | 20.1 | 40.1 | + + |
| Esterase | 2.8 | 5.5 | + |
| All six enzymes | 30.4 | 60.8 | + + |
| Inulinase + β-glucosidase | 8.2 | 16.4 | + |
| Pectinase + xylanase + β-glucosidase | 28.1 | 56.2 | + + + |
| No enzyme | + | ||
No inhibitory effect was observed with enzyme preparations in the absence of chicory extract. − no inhibitory effect; + weak inhibition; + + moderate inhibition; + + + strong inhibition; + + + + very strong inhibition
aResults were corrected by subtracting readings from the sample without treatment and the sample with enzyme only