Table 2.

Matrix of Box-Behnken design employed for optimizing size of iron nanoparticles produced by Aspergillus flavipes (MN956655.1).

Trail no	Salt*1 Conc. (mM)	Filtrate*2 volume (ml)	pH	Response (Particle size nm)
1	2.000	1.00	7.00	434
2	0.100	10.50	10.00	377
3	0.100	10.50	4.00	396
4	1.050	1.00	10.00	281
5	0.100	1.00	7.00	432
6	2.000	10.50	10.00	320
7	1.050	10.50	7.00	176
8	1.050	20.00	10.00	270
9	1.050	10.50	7.00	59
10	2.000	10.50	4.00	480
11	1.050	1.00	4.00	491
12	2.000	20.00	7.00	179
13	1.050	10.50	7.00	176
14	1.050	20.00	4.00	49
15	0.100	20.00	7.00	131

*¹FeSo₄·7H₂O.

*²Aspergillus flavipes MN956655.1 Culture filtrate.