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. 2022 Nov 23;13:1048180. doi: 10.3389/fmicb.2022.1048180

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Copyright © 2022 Chen, Wang, Zhang, Li, Gao, Nan, Zhao, Zhou and Liu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Screening of nanobodies against the hepatitis E virus (HEV) sp239 protein. (A) Alignment of the amino acid sequences of eight isolated nanobodies. The numbering of frameworks and complementarity-determining regions (CDRs) is based on previous methods (Kabat et al., 1991). (B) The specific binding between sp239 and nanobodies with indirect ELISA. The PCV2-cap protein was used as a control. (C) Titration of the nanobodies. Data were processed with triplicate experiments and are presented as the means ± SDs.