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. 2022 Nov 23;13:1048180. doi: 10.3389/fmicb.2022.1048180

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Copyright © 2022 Chen, Wang, Zhang, Li, Gao, Nan, Zhao, Zhou and Liu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Analysis of purified nanobodies. (A) His-tagged nb14 and nb53 with a predicted size of 15 kDa were detected by SDS-PAGE and Western blotting. (B) Detection of the binding activities of nb14 or nb53 to sp239 by iELISA. The PCV2-cap protein was used as a negative control. Data were processed with triplicate experiments and are presented as the means ± SDs. (C) The nb14 and nb53 interact with sp239, rHEV-239, Sar-55-239, and Kernow C1-239 protein by iELISA. The 1B5 and nb anti-PCV2 antibodies were used as positive and negative controls, respectively.