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. 2022 Nov 23;13:1048180. doi: 10.3389/fmicb.2022.1048180

FIGURE 3.

FIGURE 3

Nanobodies blocking sp239 absorption or natural swine HEV particle infection of HepG2 cells. Nanobodies blocking sp239 attachment to HepG2 cells followed by detection with Western blotting (A), immunofluorescence focus assay (IFA) (B), and flow cytometry neutralization (FCM) (C) assays. (D) Nanobodies blocking swine HEV infection of HepG2 cells by real-time RT-PCR. The nb anti-PCV2 and the MAb 1B5 served as negative and positive controls, respectively. Data are the mean ± SD of results based on triplicate experiments. P-values were calculated with student’s t-test. *P < 0.05; **P < 0.01; ***P < 0.001; ns, not significant.