Figure 2.

Dose-dependent effect of Met on 3D spheroid formation. Adherent SEM-1 cells were passaged to serum-free suspension media and tested for their ability to form 3D spheroids. Representative pictures: (A, D) untreated cells; (B, E) cells exposed to Met 1 mM for 14 days; (C, F) cells exposed to Met 5 mM for 14 days. Scale bars: 1000 µm (A-C) and 400 µm (D-F). Histograms are representative of three replicates and express the mean 3D spheroids number (diameter > 50 µm) divided by the number of cells seeded relative to untreated controls. *p < 0.05 vs untreated cells (black bar), **p < 0.05 vs cells treated with 1 mM Met (grey bar).