Figure 3.

(A) Wound healing assay was performed to determine the migratory ability of SEM-1 cells during 24 h treatment with 5 mM Met. Wound closure was quantified as the relative percentage reduction of the wound area to time 0 (T0) at 12 h and 24 h of Met treatment, using the Image J software. Scale bar: 1000 µm. Histograms are representative of three replicates. *p < 0.05 vs untreated cells (black bars) at each time point. (B) Representative immunoblots of protein factors MMP-11, IGFBP1, IGF1R. Expression levels were normalized to α-Tubulin and quantified as relative arbitrary units. *p < 0.05 vs untreated cells (black bars). (C) A representative immunoblot of truncated c-Kit isoforms is shown. (D) Transwell assay was performed to determine the invasion ability of SEM-1 cells during 24 h treatment with 5 mM Met. Extent of invasion was determined as the proportion of seeded cells that adhered to the bottom well plate. The average cell count in five microscopic fields per well was performed using the Image J software. Scale bar: 75 µm. Differences between proportions were compared using the Chi-square test. *p < 0.05 vs untreated cells.