Figure 4.

(A) Representative immunoblots of time-course experiments documenting a reduced expression of nuclear protein factors involved in cell cycle regulation (HMGA1 and cyclin D1) following 12 h and 24 h treatment with Met 5 mM in SEM-1 cells. *p < 0.05 vs untreated cells (black bars) at each time point. (B) Representative immunoblots documenting a dose-dependent reduced expression of HMGA1 and cyclin D1 following 12 h treatment with Met concentrations equal or lower than the IC₅₀ (0.5 to 5 mM). *p < 0.05 vs untreated cells (black bars). (C) Immunofluorescence assay was performed to map HMGA1 (green fluorescence) in SEM-1 cells following 12 h treatment with Met 5 mM. Nuclear staining was performed with DAPI dye (blue fluorescence). Representative confocal images. Scale bar: 75 µm. (D) Distribution of cell cycle phases by PI staining in flow cytometry following 24 h treatment with Met 5 mM. Cell distribution across the phases of cell cycle was compared using the Chi-square test. *p < 0.05 vs untreated cells.