FIGURE 2.

Rarefaction curves and UPGMA trees calculated from the five tanks. (A–D) Rarefaction curves are for OTUs and species richness (Chao1). The OTUs were analyzed using CD-HIT program at 97% sequence similarity, and the rarefaction curves and diversity indices for OTUs and Chao1 were computed using the mothur platform. (E,F) The relationships among the microbial community diversities for the five tanks. The UPGMA trees were formed using the weighted UniFrac distance. [For eukaryotic microbial communities: rarefaction curves of (A) OTUs and (C) Chao1, and (E) UPGMA tree. For prokaryotic microbial communities: rarefaction curves of (B) OTUs and (D) Chao1, and (F) UPGMA tree]. The data underlying all the graphs indicated in this figure can be found in the Supplementary Table S3.