TABLE 4.
Comparison of the bioactivity of the three isolated compounds with the reference standard compounds used in the study.
| Test compound | IC50 and IC90 values for compounds 1–3 (µM) | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| Antifungal assay | Antibacterial assay | Cytotoxicity assay | |||||||
| CA IC50 | CA IC90 | AB IC50 | AB IC90 | SA IC50 | SA IC90 | A549 IC50 | MIA PaCa-2 IC50 | PANC-1 IC50 | |
| Pestahivin (1) | 1.9 ± 0.26 | 5.6 ± 1.1 | 0.46 ± 0.06 | 1.4 ± 0.38 | > 100 | > 100 | 1.0 ± 0.27 | 0.65 ± 0.12 | > 100 |
| Pestahivin B (2) | 89 ± 17 | 144 ± 18 | 4.5 ± 0.48 | 33 ± 5.8 | > 100 | > 100 | 42 ± 5.2 | 6.2 ± 6.1 | > 100 |
| Pestahivin C (3) | 1.9 ± 0.16 | 3.4 ± 1.5 | 0.65 ± 0.07 | 1.5 ± 0.16 | > 100 | > 100 | 17.8 ± 9.7 | 5.5 ± 0.90 | > 100 |
| Gentamicin | − | − | − | − | 0.17 ± 0.02 | 0.34 ± 0.02 | − | − | − |
| Amphotericin B | 0.14 ± 0.04 | 0.16 ± 0.04 | 0.21 ± 0.02 | 0.28 ± 0.01 | − | − | − | − | − |
| Puromycin | − | − | − | − | − | − | 0.37 ± 0.06 | 0.38 ± 0.03 | 0.41 ± 0.04 |
| p-value* | 0 | 0 | 0 | 0 | − | − | 0.00001 | 0.0047 | − |
*One -way ANOVA test p < 0.05.
CA = C. albicans, AB = A. brasiliensis and SA = S. aureus.