TABLE 1.

Liquid chromatography and mass spectrometry conditions.

UPLC conditions	Parameters	Mass conditions	Parameters
Mobile phase	A: pure water with 0.1% formic acid	Ion source temperature	500
	B: acetonitrile with 0.1% formic acid
Elution gradient	0.00∼9.00 min, 5%∼95%B	Mass voltage	5500 v
	9.00∼10.00 min, 95%B
	10.00–11.10 min, 95%∼5%B
	11.10∼14.00 min, 5%B
Flow	0.35 ml/min	Gas curtain	25 psi
Temperature	40°C	Collision induced ionization	High
Injection volume	4 μl	Ion sources and modes	Turbo spray tube
			5500 V(Positive)/−4500 V(Negative )