Table 4.

The thermal stabilities and other enzymatic properties of reported thermally stable glucose dehydrogenases (GDHs).

EC	Organism	Km	kcat Vmax	melting temp (°C)	treatment temp (°C)	Halflife		Ref.
BcGDH wt
1.1.5.9	Burkholderia cepacia				65	2.5 min	30 min, 10%	5,18
					65	72 min (crosslinked)
glucose 1-dehydrogenase [NAD(P)+]
1.1.1.47	Thermoproteus tenax		202 U mg−1 specific		103	10 min		32
	Thermoplasma acidophilum		320 U mg−1 specific		75	3 h	catalytic activity is retained for 9 h at 55 °C	33
	Bacillus subtilis	293 mM	720 s−1		70		30 min, 20%	63
	Bacillus subtilis	14 mM	395 s−1	50.2				64
	Bacillus thuringiensis	0.55 mM	11.4 U mg−1		65		30 min, > 85%	65
	Bacillus thuringiensis		93 U mg−1	72.2	65	8.0 min		64
	Bacillus licheniformis		0.05 U mg−1	70.3	65	8.7 min		64
	Priestia megaterium	11 / 63 mM (pH6 /8)	320 / 820 U mg−1		65		20 min, 20%	66
	Priestia megaterium	3.1 / 12 mM (pH6 /8)	230 / 810 U mg−1		65		40 min, 70%	66
	Sulfurisphaera tokodaii		32 U mg−1 specific		80		15 min, more than 90% remaining activity, pH 8.0	34
glucose 1-dehydrogenase (NADP+)
1.1.1.119	Haloferax mediterranei	3.9 mM	550 U mg−1 specific		65	56.8 h (3 M NaCl)		35
aldose 1-dehydrogenase (NAD(P)(+))
1.1.1.359	Saccharolobus solfataricus	8.0 mM(NAD) 0.44 mM (NADP)	437 U mg−1 specific		37	40 days		36
glucose 1-dehydrogenase (PQQ, quinone)
1.1.5.2	Pyrobaculum aerophilum	680 mM	4.1 s−1		100		10 min, 100%	37
	Sorangium cellulosum	2.4 mM	1778 s−1		60		1 h, 27%	67
	Arthrobacter globiformis	0.21 mM	192 U mg−1		50		10 min, above 58%	68
	Acinetobacter calcoaceticus	24 mM	3300 U mg−1 3436 s−1		55	9.5 min	wild-type isozyme PQQGDH-B: half-life 9.5 min	69
	Acinetobacter calcoaceticus	16 mM (S415C)	3461 s−1 (S415C)		70		10 min, 90% (mutant S415C)	69
	Escherichia coli	2.8 mM	116 U mg−1		51	10 min		70
glucose 1-dehydrogenase (FAD, quinone)
1.1.5.9	Thermoascus aurantiacus	1.7 mM	~36.7 U mg−1		60		5 min, ~ 100%	71
	Thermoascus crustaceus	282 mM	~225 U mg−1	62.5	60		15 min, 75% (glycosylated)	72
	Rasamsonia emersonii	337 mM	~147 U mg−1	66.4	60		15 min, 89% (glycosylated)	72
	Aspergillus terreus	89.7 mM	541 U mg−1 specific		60	82 min		73