Table 1.
Comparison of isolated phage clusters and related phages previously described in the literature.
| Phage cluster (proposed name) | Isolation source | No. genetically diverse phages | Genome size (bp) | No. of ORFs | Host species | Related phages described in literature (nucleotide identity/coverage %) | Closest BLASTn hit31 (identity/coverage %) | Presence in obtained metavirome |
|---|---|---|---|---|---|---|---|---|
| C1 | D1-FW1, D1-FW2a | 5 | 44,341–44,655 | 54–56 |
B. uniformis P. vulgatus B. ovatus B.thetaiotamicron |
Isolated Parabacteroides phage PDS1 (MN929097.1), (95/90%)41 | BK057270.1b; 97/88% | D1-FW2 |
| C2 (Tigiphages) | D1-FW1, D1-FW2 | 7 | 33,659–33,821 | 46 |
B. uniformis P. vulgatus B. ovatus B.thetaiotamicron |
First described and isolated in this studyc | BK033007.1b; 90/92% | D1-FW2 |
| C3 (Meggiephages) | D2-FW2 | 9 | 74,237–74,722 | 117 | B. uniformis | First described and isolated in this study | BK032629.1b; 97/80% | D2-FW2 |
| C4 (CrAss-like phages) | D2-FW2 | 2d | 99,030–100,005 | 103 | B. uniformis | Isolated CrAss-like phages ΦcrAss00112, ΦcrAss00214; DAC15 and DAC1713; (77/24%—ΦcrAss001) | BK035398.1b; 99/89% | D2-FW1, D2-FW2, D1-FW2 |
| C5 | D2-FW2 | 1 | 42,552 | 54 |
B. uniformis P. vulgatus |
Isolated temperate phage of Bacteroides dorei: Bacteroides phage p00 Hankyphage15; (99/99%) | BK010646.1; 99/99% | D2-FW1, D2-FW2 |
| C6 | D1-FW1, D1-FW2 | 2d | 73,875 | 107 |
B. uniformis P. vulgatus |
Isolated in this study, identical to described metagenomics putative phage LoVEphage DEVoC_Healthy_0555 (MZ919987)42 (99.27 /94%) |
MZ919987.1b; 99/94% | D1-FW2 |
| C7 (Bacuni phages) | D1-FW1, D1-FW2 | 2 | 40,421–40,744 | 50–51 |
B. uniformis P. vulgatus B. ovatus |
Bacuni phages F1, F2 and F419 (99/99%) | MT635598.1; 99/99% | D1-FW2 |
aD, donor; FW, fecal water.
bAssembled putative viral contig from metagenomic data.
cApp. 57% amino acid identity in 20% of ORFs with B. thetaiotaomicron infecting alpha cluster phages from13.
dPartial viral genomes in cluster.