Table 1.
Measured binding affinities of rHSA and AlproTox variants to hFcRn (KD, Kon and Koff) at pH 5.5.
| KD (M) | Kon (1/ms) | Koff (1/s) | R2 1:1 fit | X2 fit | |
|---|---|---|---|---|---|
| Albumin NB | – | – | – | – | – |
| Albumin WT | 2.1E-07 | 2.8E + 04 | 5.8E-03 | 0.979 | 0.788 |
| Albumin HB | 7.7E-09 | 2.5E + 04 | 1.83E-04 | 0.997 | 0.865 |
| AlproTox NB | – | – | – | – | – |
| AlproTox WT | 1.1E-08 | 1.8E + 04 | 1.96E-04 | 0.998 | 0.853 |
| AlproTox HB | 5.3E-09 | 5.0E + 04 | 2.6E-04 | 0.9982 | 0.459 |
Data were obtained by Bio-Layer Interferometry using an Octet system. Sensors were coated with hFcRn and lowered into wells containing the analyte. For affinity analyses, the sensorgrams were fitted to a 1:1 model.