FIGURE 3.

MDP reduces DSS-induced intestinal barrier damage. (A) Mice were fasted overnight on Day 7 and given FITC-Dextran by gavage on Day 8. After 4 h, blood was taken from the eyeball from light. The serum was separated and analyzed for the detection of intestinal permeability (n = 6, each group). (B) Western blotting analysis for ZO-1 and E-cadherin with β-Actin as the internal standard protein in the colon of PBS, MDP, DSS + PBS, and DSS + MDP group mice. Representative images of three duplicate samples of the immune blotting were shown. (C,D) Quantification of the relative expression of ZO-1 and E-cadherin in panel (B) by ImageJ, respectively. Data were expressed as mean ± SEM. *p <0.05, **p <0.01, ****p <0.0001, ns, not significant, One-way ANOVA.