Table 1.

Comparison of culture growth parameters from closed batch experiments.^*

Closed batch	Agitation		OD578		μL [h−1]	GTL [h]
	Method	[rpm]	Max	time [h]
SB	Shaker	100	0.9292	301	0.07	26.93
	Shaker.2	100	1.1929	325	0.25	7.87
	Stirrer	100	1.1089	325	0.29	6.8
	Stirrer	500	1.2339	323	0.95	2.11
	Stirrer/2×	500	1.2924	179	0.94	2.12
SCB	Shaker	100	1.0819	325	0.21	9.65
	Shaker	180	1.0530	209	0.65	3.06
	Shaker/2×	100	1.0561	291	0.48	4.19
	Stirrer	100	0.4766	326	0.12	16.63
	Stirrer	500	0.9322	321	0.27	7.37
	Stirrer	800	0.9318	158	0.24	8.48
	Stirrer	1,100	0.6804	209	0.15	13.74
	Stirrer	1,400	0.1691	96	–	–

^*Closed batch SB und SCB experimental groups are listed with their respective agitation method and intensity (rpm). Maximum (max) measured OD₅₇₈ and the respective needed time are compared. Highest recorded μ_L and GT_L were calculated according to Formulas 1 and 3. shaker.2, an experimental group, subjected to the same cultivation conditions after inoculation transfer to a new culture; /2×, double H₂/CO₂ feeding timepoints.