Table 2.
Distinct experimental groups of 1.5 L reactor cultures (Figure 3). **
| Phase | Runs [N] | n | Inoculum | Main goal | Successful/total | ||
|---|---|---|---|---|---|---|---|
| OD578 | g | Runs | Time [h] | ||||
| 1 | 5 | 5 | Stat. | 10,000 | Define distinct agitation ramps | 6/18 | 612/1552 |
| 2 | 3 | 7 | Stat. | 4,000 | Reproduce ramps | 3/4 | 308/399 |
| 3 | 3 | 8 | Exp.* | 3,000 | Reproduce ramps and test pipeline | 3/3 | 303/303 |
| 4 | 1 | 3 | Late exp. (2.1) | Reproduce best ramp | 2/2 | 107/107 | |
| 5 | 1 | 3 | Late exp. (1.8) | Reproduce best ramp + rpm and vvm | 2/2 | 94/94 | |
| 6 | 1 | 2 | Exp. (1.3) | Reproduce best ramp + rpm and vvm - OD | 2/2 | 100/100 | |
| 7 | 1 | 3 | Exp. (1.5) | Reproduce best ramp -OD | 2/2 | 79/79 | |
**Overview of experimental setup of 1.5 L reactor cultures from Figures 3A–F: Phases; number of runs (N); bioreactor replicates (n); inoculum stage of growth (stat., stationary; exp., exponential), optical density (OD578), centrifuge forces (g); main goals of each phase; number of successful/total runs and cultivation time in h. +rpm and vvm: increase of agitation and supply of gaseous substrate; +rpm and vvm-OD: increase of agitation and supply of gaseous substrate and decrease of OD578 of inoculum; *exponential only in Figure 3E, not in Figures 3A,B.