Table 3.
Comparison of growth parameters from experiments with 1.5 L reactor cultures.***
| Replicate ID [Phase.run. replicate] | Ramp | OD578 | μ [h−1] | GT [h] | Wet biomass [g L−1] | |
|---|---|---|---|---|---|---|
| Max | Time [h] | |||||
| 1.9.3 | Ad/man | 1.8452 | 142 | 0.11 | 6.08 | 5.17 |
| 1.10.2 | Cont-cons | 1.7652 | 107 | 0.12 | 5.78 | 7.72 |
| 1.10.4 | Cont-prog | 1.3007 | 0.10 | 6.72 | 4.28 | |
| 1.12.1 | Step-cons | 2.6770 | 90 | 0.06 | 11.24 | – |
| 1.12.2 | Step-prog | 1.1111 | 69 | 0.06 | 12.58 | – |
| 2.19.2 | Ad/step-man | 0.4430 | 72 | 0.06 | 11.43 | – |
| 2.19.3 | 0.4180 | 0.04 | 16.82 | – | ||
| 2.19.4 | 0.8757 | 99 | 0.10 | 7.04 | – | |
| 2.20.1 | Step-prog | 1.4310 | 65 | 0.09 | 7.02 | – |
| 2.20.2 | 2.2980 | 69 | 0.08 | 8.86 | – | |
| 2.21.3 | Step-cons | 3.3884 | 114 | 0.06 | 11.20 | 3.52 |
| 2.21.4 | 3.2096 | 0.09 | 7.86 | – | ||
| 3.23.1 | Ad/step-man | 2.2960 | 116 | 0.10 | 7.10 | – |
| 3.23.2 | 2.4864 | 109 | 0.08 | 8.78 | 4.09 | |
| 3.23.3 | 2.3598 | 0.08 | 8.62 | 5.96 | ||
| 3.23.4 | 2.3122 | 0.10 | 7.12 | 6.27 | ||
| 3.24.2 | Cont-cons | 2.1163 | 90 | 0.06 | 12.58 | – |
| 3.24.4 | 1.1018 | 0.05 | 13.82 | – | ||
| 3.25.1 | Cont-prog | 2.2690 | 76 | 0.06 | 12.36 | 3.39 |
| 3.25.3 | 2.4040 | 0.07 | 9.84 | 5.39 | ||
| 4.27.2 | Step-cons | 2.1700 | 66 | 0.11 | 6.34 | – |
| 4.27.3 | 2.8588 | 91 | 0.13 | 5.53 | – | |
| 4.27.4 | 1.8230 | 66 | 0.16 | 4.30 | – | |
| 5.29.1 | Step-cons +rpm and vvm | 2.1670 | 70 | 0.07 | 10.20 | – |
| 5.29.2 | 2.4113 | 94 | 0.06 | 12.54 | – | |
| 5.29.4 | 1.7824 | 86 | 0.04 | 17.78 | – | |
| 6.31.1 | Step-cons +rpm and vvm -OD | 2.0025 | 76 | 0.09 | 8.02 | – |
| 6.31.2 | 2.3625 | 90 | 0.09 | 7.30 | – | |
| 7.33.1 | Step-cons. -OD | 3.086 | 74 | 0.16 | 4.44 | – |
| 7.33.2 | 3.1852 | 74 | 0.14 | 4.91 | – | |
| 7.33.3 | 3.3818 | 77 | 0.14 | 4.89 | – | |
Individual reactor replicates are listed with their respective ID ([phase.run.replicate] numbers) and tested agitation ramp (ad: adaptation, man; manual, cont-cons: continuous conservative, cont-prog: continuous progressive, step-cons: stepwise conservative, step-prog: stepwise progressive), +rpm and vvm: increase of agitation and gaseous substrate supply, -OD: decrease of OD of inoculum. For each run a highest recorded (max) OD578 and the corresponding time are listed. Specific growth rate (μ) and generation time (GT) were calculated according to Formulas 2 and 4 and compared. If biomass was harvested, the measured weight in grams (g) of wet pellets is presented in the last column on the right. IDs and noteworthy values are matching the color code of respective growth curves from Figure 3.