Table 1.
Brightness comparison for FCS measurements.
| Sample | Brightness (CPSM) | Reduced X2 | n |
|---|---|---|---|
| Cytosolic eGFP | 4500 ± 700 | (14 ± 7) × 10–7 | 7 |
| Monomeric CD-86/eGFP | 4600 ± 900 | (24 ± 9) × 10–7 | 6 |
| Tandem CD-86/eGFP/eGFP | 8300 ± 900 | (20 ± 9) × 10–7 | 6 |
| Full-length hZIP4/eGFP | 8100 ± 1800 | (16 ± 7) × 10–7 | 6 |
| Truncated hZIP4/eGFP | 8200 ± 2000 | (10 ± 5) × 10–7 | 6 |
Calculated brightness of molecules tagged with eGFP for HEK293 cells expressed cytosolic, membrane-bound monomeric eGFP control, membrane-bound dimeric eGFP control, full-length hZIP4/eGFP, and truncated hZIP4/eGFP protein, respectively. Brightness values are reported as counts per second per molecule (CPSM). X2 refers to the goodness of fit of the data to the two-component model. Data represent the mean ± the standard error of the mean for the number of cells examined (n).