Table 1.
Bacteria and plasmids used in this work.
| Strain or plasmid | Relevant characteristic(s) | Reference or source |
|---|---|---|
| P13 | The original strain of L. amnigena P13 | This study |
| DH5α | Escherichia coli DH5α was used as a host for cloning of the blaLAQ-1 gene | Our laboratory collection |
| BL21 | E. coli BL21 was used as a host for expression of LAQ-1 | Our laboratory collection |
| ATCC 25922 | E. coli ATCC 25922 was used as a quality control for antimicrobial susceptibility testing | Our laboratory collection |
| pUCP24-blaLAQ-1/DH5α | DH5α carrying the recombinant plasmid pUCP24-blaLAQ-1 | This study |
| pET-28a-blaLAQ-1/BL21 | BL21 carrying the recombinant plasmid pET-28a-blaLAQ-1 | This study |
| pUCP24 | Cloning vector for the PCR products of the blaLAQ-1 gene with its upstream promoter region, GENr | Our laboratory collection |
| pET 28a | Expression vector for the PCR products of the ORF of the blaLAQ-1 gene, KANr | Our laboratory collection |
rResistance; GEN, gentamicin; KAN, kanamycin.