Skip to main content
. 2022 Nov 23;13:990736. doi: 10.3389/fmicb.2022.990736

Table 1.

Bacteria and plasmids used in this work.

Strain or plasmid Relevant characteristic(s) Reference or source
P13 The original strain of L. amnigena P13 This study
DH5α Escherichia coli DH5α was used as a host for cloning of the blaLAQ-1 gene Our laboratory collection
BL21 E. coli BL21 was used as a host for expression of LAQ-1 Our laboratory collection
ATCC 25922 E. coli ATCC 25922 was used as a quality control for antimicrobial susceptibility testing Our laboratory collection
pUCP24-blaLAQ-1/DH5α DH5α carrying the recombinant plasmid pUCP24-blaLAQ-1 This study
pET-28a-blaLAQ-1/BL21 BL21 carrying the recombinant plasmid pET-28a-blaLAQ-1 This study
pUCP24 Cloning vector for the PCR products of the blaLAQ-1 gene with its upstream promoter region, GENr Our laboratory collection
pET 28a Expression vector for the PCR products of the ORF of the blaLAQ-1 gene, KANr Our laboratory collection

rResistance; GEN, gentamicin; KAN, kanamycin.