FIGURE 2.

(A) Absorption and (B) emission spectra of 5 μM CS-ALP in the presence of different concentrations of ALP. (C) Calibration curves. (D) Fluorescence intensity at 716 nm vs. the reaction time with varying levels of ALP activity. The tests were carried out in aqueous solution (5% DMSO TBS buffer solution, pH 8.0) after incubation at 37°C for 0.5 h. λ _ex = 680 nm.