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. 2022 Nov 23;13:1043945. doi: 10.3389/fphar.2022.1043945

FIGURE 3.

FIGURE 3

Mefunidone abated renal apoptosis (A) TUNEL staining in the renal tissues on day 2 after IRI modeling (×400). (B) Quantification of TUNEL positive cell count per scope. (C) Western blot analysis and quantitative data of cleaved-caspase3 in the renal tissues on day 2 after IRI modeling. (D,E) Flow cytometry and quantitative comparison were applied to detect apoptosis of HK-2 cells in H/R model with or without mefunidone. (F) Western blot analysis and quantitative data of cleaved-caspase3 in the H/R model of HK-2 cells with or without mefunidone. Dose of mefunidone in mice: 100 mg/kg; Dose of mefunidone in HK-2 cells: 80 μg/ml. Data represent mean ± SEM (n = 3–5). *p < 0.05, vs. Sham group; **p < 0.01, vs. Sham group; ***p < 0.001, vs. Sham group; # p < 0.05, vs. Modeling group; ## p < 0.01, vs. Modeling group; ### p < 0.001, vs. Modeling group.