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. 2022 Dec 6;42(12):BSR20221165. doi: 10.1042/BSR20221165

Figure 3. IL-33 induces MSK1 activation and phosphorylation of CREB.

Figure 3

(A) Schematic of the murine granzyme C locus showing the five exons. A consensus-binding site for CREB (underlined text) is located 54bp of the transcriptional start site annotated in Ensembl (transcript ENSMUST00000015585.4). (B) Wild-type BMMCs were incubated with either 2 μM PD184352 or 1 μM VX745 or 10 μM SB 747651A as indicated for 1 h before stimulation with 10 ng/ml IL-33 for 30 min. The levels of phospho-ERK1/2, total ERK1/2, phopho-p38, total p38, phospho-MSK1, total MSK1 phospho-CREB, total CREB, and GAPDH were determined by immunoblotting.