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. 2022 Nov 23;13:942061. doi: 10.3389/fphar.2022.942061

FIGURE 2.

FIGURE 2

Apol9a is increased in myogenic differentiation and decreased in obese mice. RNA sequencing (RNA-seq) data of C2C12 cells with or without myogenic induction were collected. (A) Representative images from C2C12 cells in growth medium (GM) and differentiation medium (DM). Scale bar, 100 μm. (B) Volcano plot showing differentially expressed genes (DEGs) in differentiated or undifferentiated C2C12 cells. (C) Ten most upregulated GO terms during C2C12 differentiation according to the RNA-seq data. (D) Expression profiles of apolipoprotein family genes during C2C12 differentiation. (E) The relative mRNA expression (left) and protein levels (right) of Apol9a during C2C12 differentiation were measured using qPCR and western blotting. (F) The relative mRNA expression (left) and protein levels (right) of Apol9a in obese skeletal muscle were measured through qPCR and western blotting. The data are presented as mean ± SEM from at least three separate experiments. *p < 0.05, **p < 0.01, ***p < 0.001.