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. 2022 Nov 23;13:942061. doi: 10.3389/fphar.2022.942061

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Copyright © 2022 Jiang, Ji, Cui, Wang, Wang, Chen and Zhu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Apol9a overexpression promotes myogenic differentiation. C2C12 cells were induced to differentiate after pcDNA3.1 or pcDNA3.1-Apol9a transfection for 4 days. (A) qPCR and western blot validation of the efficiency of Apol9a overexpression in C2C12 cells. (B) Morphology of C2C12 cells treated with pcDNA3.1 or pcDNA3.1-Apol9a. The results of quantification of average myotube diameter are shown in the right. (C) Protein expression levels of MyHC, MyoD, Myf5, MyoG, p-ERK 1/2, ERK 1/2, and β-actin were estimated through western blot analysis after transfection. (D) Gray scale analysis of western blot results of (C) determined using ImageJ software. The data are expressed as mean ± SEM from at least three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001 vs. pcDNA3.1 group.