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. 2022 Nov 23;13:942061. doi: 10.3389/fphar.2022.942061

FIGURE 6.

FIGURE 6

Transcriptomic data. C2C12 cells with or without si-Apol9a treatment was harvested after myogenic differentiation, and then transcriptomic analysis was performed. (A) Principal component analysis of RNA-seq data from C2C12 cells with (si-Apol9a) or without (si-NC) Apol9a knockdown after myogenic differentiation (n = 3 per group). (B) Volcano plot showing the DEGs in C2C12 cells with (si-Apol9a) or without (si-NC) Apol9a knockdown after myogenic differentiation. (C) Gene ontology analysis of upregulated DEGs. (D) Western blot analysis showed the protein levels of p-P38, P38, p-JNK, JNK, p-ERK1/2, and ERK1/2. (E) Gray scale analysis of western blot results determined using ImageJ software. (F) Western blot analysis for p-ERK1/2 and ERK1/2. C2C12 cells were cultured in GM or DM for 4 days. The data are expressed as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001 vs. si-NC group.