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. 2022 Nov 23;13:942061. doi: 10.3389/fphar.2022.942061

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Copyright © 2022 Jiang, Ji, Cui, Wang, Wang, Chen and Zhu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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ERK inhibitor reverses Apol9a knockdown-induced inhibition of myogenesis. C2C12 cells were transfected with NC or Apol9a siRNA. After 12 h, the GM was replaced with the DM to induce differentiation or DM containing PD98059 (50 μm). (A) Morphology of C2C12 cells treated with si-NC, si-Apol9a, and si-Apol9a plus PD98059 (50 μm). The results of quantification of average myotube diameter are shown in the right. (B) Protein expression levels of MyHC, MyoD, MyoG, p-ERK1/2, and ERK1/2 as detected through western blot analysis. Gray intensity analysis is shown on the right. (C) Representative images of immunofluorescence for MyHC (green) are shown. The fusion index (%) was quantified and is shown on the right. Scale bar, 100 μm. Each experiment in this study was repeated at least three independent times. *p < 0.05, **p < 0.01, ***p < 0.001.