Figure 5.

DEX-induced GR binding at a putative GILZ enhancer is reduced after IFNα pre-treatment. (A) DHS linkage predictions are mapped to indicate potential looping regulatory regions connected to the GILZ promoter (19). (B) ChIP-seq data of STAT1 binding in CD14+ monocytes treated with IFNγ (GSE99887), H3K27Ac histone acetylation and GR binding in THP1 cells treated with 100 nM DEX (GSE43036) and GR binding in SUP-B15 cells treated with 100 nM DEX (GSE107584). (C–F) ChIP for GR in L363 cells treated with DEX or IFNα and DEX. Enrichment was assessed in regions (C) A, (D) Z,(E) K and (F) L as indicated in figure B. n=3 from three independent experiments. ns, Not significant.