FIGURE 2.

AA147 improved neurological outcomes and decreased neuronal death post-cardiac arrest and cardiopulmonary resuscitation (CA/CPR). Mice were subjected to sham or CA/CPR surgery, with vehicle or AA147 administered 1 day before the surgery and 15 min after the return of spontaneous circulation (ROSC). The effect of AA147 on improving neurological function and reducing neuronal death was evaluated at 3 day after CA/CPR. AA147-treated mice scored significantly higher in the neurologic scoring system: the 9-point scoring system (vertical screen, horizontal bar, and rope) (A), and had longer travel distance and active time in the open field test (B). However, there was no significant difference in the improvement of cognitive function between group CA + Vehicle and CA + AA147, presented in Y-maze. (C) Data are presented as mean ± SD (n = 5–7). (D) Representative HE staining images of the hippocampal cornu ammonis 1 (CA1) region and (E) counts of dead CA1 neurons. Black arrows indicate the normal pyramidal cells with intact morphology, while red arrows show the dead neurons with shrunken profiles and pyknotic and deeply-stained nuclei. Scare bar = 20 μm. Data are displayed as mean ± SD (n = 3). (F) Representative images of FJB staining and (G) the number of FJB⁺ neurons in the hippocampal CA1 region of each group. White arrows show the FJB⁺ neurons stained with green fluorescence, indicating neuronal death. Scare bar = 20 μm. Data are displayed as mean ± SD (n = 3). *p < 0.05. **p < 0.01. ***p < 0.001.