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. 2022 Nov 23;13:1001210. doi: 10.3389/fendo.2022.1001210

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Copyright © 2022 Tratwal, Falgayrac, During, Bertheaume, Bataclan, Tavakol, Campos, Duponchel, Daley, Penel, Chauveau and Naveiras

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Heterogeneity in the formation of lipidated adipocytes upon induction of adipogenesis by confluency and serum exposure (spontaneous) versus DMI cocktail (induced). OP9 cells were (A–D) passaged subconfluently and remained undifferentiated on day 17 of culture, (B, E, H) differentiated spontaneously upon confluency in the presence of serum for 17 days, or (E, F, I) induced with a classical adipogenic differentiation cocktail in the same serum-containing conditions after confluent plating and 17 days in culture. (G) Oil red O measurements show the highest adipogenic differentiation in the induced condition at day 17 in culture. **P<0.01 by student’s t-test. Error bars represent mean ± s.d. (n = 3 biological replicates). DMI: 1µM dexamethasone, 0.5mM isobutyl-methylxanthine, 5µg/ml insulin.