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. 2022 Nov 23;13:1001210. doi: 10.3389/fendo.2022.1001210

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Copyright © 2022 Tratwal, Falgayrac, During, Bertheaume, Bataclan, Tavakol, Campos, Duponchel, Daley, Penel, Chauveau and Naveiras

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Population level differences in lipid composition are more marked than differences in the canonical adipocytic transcriptional signature for spontaneous versus induced cultures. (A) RT-qPCR markers of adipogenesis are increased in induced OP9-adipocytes. (B) Desaturase expression is highest in day 17 induced OP9-adipocytes while one of the elongases in fatty acid de novo synthesis is decreased. ****P < 0.0001, ***P < 0.001, **P < 0.01, *P < 0.05 by Bonferroni’s multiple comparisons test. Error bars represent mean ± s.d. (n = 3 independent experiments). (C–G) HPLC analysis of spontaneously differentiated or induced OP9 adipocytes revealed (C) nuances of the most abundant saturated, mono-, and polyunsaturated lipid species, with (D) overall total higher unsaturation content in induced versus spontaneous conditions. (E) Percentage of individual fatty acid species reveals (F) a greater 16:1/16:0 unsaturation ratio in the induced condition. (G) Unsaturation ratios 18:1/18:0 were not significantly different. ****P < 0.0001, ***P < 0.001, **P < 0.01, *P < 0.05 by ANOVA test with Bonferroni’s multiple comparisons adjustment. Error bars represent mean ± s.d. (n = 3 independent experiments). HKGs; housekeeping genes.