Figure 3.

Raman microspectroscopy detects unsaturated spectra at the single lipid droplet level in spontaneous and induced adipocytes. (A) An optical image of an adipocyte in the induced condition (iOP9) analyzed by Raman imaging. (B) Raman image of the scores of PC1 in induced condition. Pixels in black represent a score of PC1 equal to zero, which corresponds to the absence of PC1. Pixels in shades of blue correspond to the contribution of PC1. (C) Loading PC1 is characteristic of a spectrum of unsaturated lipid-rich and captures more than 95% of the variation. (D) An optical image of an adipocyte in spontaneous condition (sOP9) analyzed by Raman imaging. (E) Raman image of the scores of PC1 in spontaneous condition. Pixels in black represent a score of PC1 equal to zero, which corresponds to the absence of PC1. Pixels in shades of red correspond to the contribution of PC1. (F) Loading PC1 is characteristic of a spectrum of saturated lipid-rich. The Raman images of the scores and the loadings of PC2 and PC3 capture less than 2% and are presented in supplementary files ( Figure S4 ).