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. 2022 Nov 23;13:1001210. doi: 10.3389/fendo.2022.1001210

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Copyright © 2022 Tratwal, Falgayrac, During, Bertheaume, Bataclan, Tavakol, Campos, Duponchel, Daley, Penel, Chauveau and Naveiras

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Induced adipocytes are composed of larger lipid droplets than spontaneous adipocytes, but there is no overall correlation between lipid droplet diameter and unsaturation ratio. (A, B) Unsaturation ratio as a function of the diameter of the LDs for sOP9 versus iOP9 adipocytes (n = 3 independent experiments, pooled for LD analysis). The low R2 for both conditions indicates that the diameter of the LDs is not correlated with the unsaturation ratio. Dashed lines represent the correlation line. The Y axis is colored as function of the score of PC1. The circles in dark blue corresponds to LDs identified as unsaturated rich. The circles in dark red correspond to LDs identified as saturated-rich. The circles in yellow and light blue correspond to LDs identified as mixture. (C) Histogram for frequency of LDs as a function of the diameter for the spontaneous and induced condition. Vertical lines show the mean diameter of the LD for each condition. The mean diameter of LDs is 5.24µm (±1.85µm) and 7.87 µm (±3.83µm) for sOP9 adipocytes and iOP9 adipocytes, respectively. The LDs mean diameter of sOP9-adipocytes is significantly lower compared to iOP9-adipocytes (P < 0.0001, Mann-Whitney test). (D, E) When separated according to LD type a trend toward larger unsaturated lipid droplets in the induced condition is observed. (F) Unsaturation ratios of sOP9 versus iOP9 at LD level. LDs from iOP9 adipocytes have a higher mean unsaturation ratio compared to LDs from sOP9 adipocytes. (P < 0.0014, Mann-Whitney test). (G) At single droplet resolution, larger droplets (>10µm) have a higher unsaturation ratio as compared to smaller lipid droplets, while smaller droplets (<10µm) in induced OP9-adipocytes (n = 1973) have a higher unsaturation ratio than those of spontaneous OP9-adipocytes (n = 2579). Highest unsaturation ratio overall is seen in large droplets from spontaneous OP9-adipocytes (n = 41). (H) Unsaturation ratio as a function of the condition (spontaneous and induced) and the nature of the lipid (saturated-rich, unsaturated-rich and mixture). *P < 0.05, ****P < 0.001 by Kruskal-Wallis test. The lipid droplet of spontaneous unsaturated-rich have the higher unsaturation ratio compared to induced unsaturated-rich. **p<0.01.