Figure 6.

Short-term co-culture of OP9-adipocytes with hematopoietic stem and progenitor cells favours hematopoietic expansion in the spontaneous condition. (A) Total hematopoietic cell expansion of 2000 initial HSPCs after seven days in co-culture (including cells in adherent and cells in suspension) is significantly higher (p < 0.0001) when hematopoietic stem and progenitor cells, HSPCs, (cKit⁺Lin^-Sca1⁺, KLS) are co-seeded with spontaneous- compared to induced OP9 adipocytes. (B) The colony forming capacity of HSPCs seeded with spontaneous OP9 adipocytes is significantly higher (P < 0.05) than when cultured with induced OP9 adipocytes. (C), Total hematopoietic expansion (in the absence of stroma) after two-day co-culture of 2000 HSPCs in conditioned medium from OP9 cultures comprising increasing ratios of conditioned-to-fresh media. ****P < 0.0001, **P < 0.01, *P < 0.05 by Bonferroni’s multiple comparisons test. Error bars represent mean ± s.d. (n = 3 biological replicates).