Figure 7.

HPLC fatty acid quantification of primary human BM samples confirms a higher unsaturation ratio in constitutive BMAd-rich marrow (femoral head) versus regulated BMAd-rich marrow (ileac crest post-chemotherapy). (A–C) HPLC analysis of lipids isolated from human iliac crest bone marrow aspirates after induction chemotherapy (regulated site, labile marrow) or from the bone marrow of femoral specimens collected upon hip replacement surgery (constitutive site, stable marrow). (A) Higher unsaturation content in stable versus labile marrow oil reveal nuances of individual saturated, mono-, and polyunsaturated lipid species. (B, C) Greater unsaturation ratios in the human marrow from the constitutive site versus the regulated siten. (D) **P < 0.01, *P< 0.05 by student’s t-test. Error bars represent mean ± s.d. (n = 4 experiments from independent donors). Regulated site (iliac crest): 3 females, 1 male (age = 55 ± 12 years). Constitutive site (hip replacement): 3 males, 1 female (age = 68 ± 10 years).