Figure 3. Sequence alignment of tRNAIle reveals a high-degree of conservation of T4272 and A4316 nucleotides across species. (A) The nucleotides T at the position 4272 and A at position 4316 are highly conserved among species. (B) Prediction of the structural and folding changes due to T4272C and A4316G nucleotides in tRNAIle using the Mfold program. The free energy change between the mutant and wild-type states was determined.
