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Cloning and identification of the ZmnMAT1 gene. (A) Structure of the ZmnMAT1 gene and positions of Mu insertion (triangle) in zmnmat1 mutant. (B) The ZmnMAT1 protein contains a RT motif (a reverse transcriptase domain) and an X motif (an RNA binding and splicing domain). (C) qRT-PCR analysis of ZmnMAT1 expression in 10 DAP kernels of zmnmat1 mutant and WT siblings. Expression level was normalized against ZmActin. Values are shown as the average ± S.E. (* p< 0.05, Student’s t-test).
