Figure 4.

The expression profile of ZmnMAT1 and subcellular localization of ZmnMAT1 protein. (A) Expression profiles of ZmnMAT1 in various tissues and during maize kernel development. ZmActin was used as an internal control. Data was shown as the average ± S.E. of three biological replicates. (B) Subcellular localization of 35S:ZmnMAT1-YFP fusion protein in tobacco leaf epidermis cells. MitoTracker Red was used to label mitochondria. Scale bar =50 μm. (C) Subcellular localization of 35S:ZmnMAT1-YFP fusion protein in Arabidopsis thaliana root hairs. MitoTracker Red was used to label mitochondria. Scale bar =50 μm. Fluorescence of YFP and MitoTracker were detected with excitation at 514 and 579 nm, respectively. And the master gain of YFP and MitoTracker were 731 and 500, respectively.