Figure 5.

The loss of Nad1 and Nad4 mature transcripts and splicing deficiency of Nad1 intron 1 and Nad4 intron 2 in zmnmat1 mutant. (A) RT-PCR analysis of 35 mitochondria-encoded transcripts in 10 DAP WT (left) and zmnmat1 mutant (right) kernels. ZmActin was used as internal control. (B) RT-PCR analysis of Nad1 intron-splicing efficiency in WT and zmnmat1 mutants (left) and schematic structure of Nad1 gene (right). The expected amplification products using different primer pairs are indicated (right). Red asterisks indicate the abnormal unspliced fragments and red triangles indicate the normal spliced fragments (left). (C) RT-PCR analysis of Nad4 intron-splicing efficiency in WT and zmnmat1 mutants (left) and schematic structure of Nad4 gene (right). The expected amplification products using different primer pairs are indicated (right). Red asterisks indicate the abnormal unspliced fragments and red triangles indicate the normal spliced fragments (left).