Figure 6.

Splicing deficiency of Nad1 intron 1 and Nad4 intron 2 in 10 DAP zmnmat1 kernels. (A) qRT-PCR analysis of intron splicing deficiency of mitochondrial genes. Primers spanned adjacent exons and introns were used to measure the differences in splicing efficiency. Data was shown as the average ± S.E. of three biological replicates. (B) qRT-PCR analysis of mature mitochondrial transcripts. Primers spanning adjacent exons were used for measuring differences in each spliced fragment. ZmActin was used as an internal control. Data was shown as the average ± S.E. of three biological replicates.