Figure 7.

Impacted mitochondrial complexes in zmnmat1 kernels at 10 DAP. (A) BN-PAGE analysis of mitochondrial complexes. The positions of complex I+III₂, complex I, complex III and complex V were indicated. About 100 μg of mitochondrial protein was loaded in each lane. (B) In-gel NADH dehydrogenase activity test of complex (I) The positions of complex I+III₂ and complex I was indicated. About 100 μg of mitochondrial protein was loaded in each lane. (C) Western blot analysis with antibodies against NAD9 (subunit of complex I), Cytc (Cytochrome c), COX2 (subunit of complex IV), ATPase-B (subunit of complex V) and AOX2 (stands for alternative oxidase) in mitochondrial protein from WT and zmnmat1 immature seeds at 10 DAP. Tubulin was used as a loading control. About 25 μg of mitochondrial protein was loaded in WT and zmnmat1 lanes, and about 12.5 μg of mitochondrial protein was loaded in ½WT lane.