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. 2022 Nov 23;13:1033869. doi: 10.3389/fpls.2022.1033869

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Copyright © 2022 Fan, Fu, Wei, Jia, Zhao, Wang, Cao, Liu, Ren and Liu

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Expression of Aox1, Aox2, and Aox3 in the zmnmat1 mutant. (A) RT-PCR analysis of Aox1, Aox2, and Aox3 expression in the 10 DAP WT and zmnmat1 kernels. The expression level was normalized against ZmActin. (B) qRT-PCR analysis of Aox1, Aox2, and Aox3 expression using ZmActin as an internal control. Data is the average ± S.E. of three biological replicates. (C-F) Transmission electron microscopy image of the WT (C, D) and zmnmat1 mutant (E, F) in 15 DAP endosperm cells. (C, E) Low magnification. Scale bar=5 μm. (D, F) High magnification. Scale bar=2 μm. Red arrows indicate the mitochondria.