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. 2022 Nov 23;13:1033869. doi: 10.3389/fpls.2022.1033869

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Copyright © 2022 Fan, Fu, Wei, Jia, Zhao, Wang, Cao, Liu, Ren and Liu

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Y2H analysis of the interaction between ZmnMAT1 with factors involved in the splicing of Nad1 intron 1 and/or Nad4 intron 2. The Y2H strains harboring the indicated bait and prey constructs were spotted on SD-Trp-Leu (DDO) and SD-Trp-Leu-His-Ade (QDO).The interaction between T-antigen and Human P53 was used as a positive control. The interaction between T-antigen/Lam and BD-ZmnMAT1/AD were used as negative controls. AD, activation domain; BD, binding domain.