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. 2022 Nov 23;12:1043177. doi: 10.3389/fonc.2022.1043177

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Copyright © 2022 Jia, Li, Wang, Feng, Pan, Lin, Song, Yang and Yang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Knockdown of ZNF24 reduces the level of SLC7A5 protein. (A–D) The regulation between ZNF24 and SLC7A5 was analyzed by qRT-PCR and Western blot, and ZNF24 positively regulated the protein level of SLC7A5. (E) CHX assay showed that ZNF24 knockdown shortened the half-life of SLC7A5 protein synthesis. (F) Western blot showed that SLC7A5 was expressed in the cytoplasmic membrane and nuclear of A549 and H358, while ZNF24 was mainly expressed in the nucleus. (G) Extract nuclear protein and Western blot showed that knockdown of ZNF24 inhibited the expression of SLC7A5 in the nucleus. (H, I). Immunofluorescence assay showed that SLC7A5 expression was decreased after ZNF24 was knocked out. The experiments were repeated 3 times. Data are shown as means ± SD. P values were calculated with two-tailed Student’s t-test, *p < 0.05, **p < 0.01, ***p < 0.001.