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. 2022 Nov 23;13:1043464. doi: 10.3389/fpls.2022.1043464

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Copyright © 2022 Ma, Chen, Liu, Xue, Zhang, Xu, Lai and Lin

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Transcription factors (TFs) regulate DlXTH23.5 and DlXTH25 expression. (A) TFs in the DlXTH23.5 and DlXTH25 relationship prediction diagram. (B) Expression profile of TFs during early somatic embryogenesis (SE). (C) Expression profiles of DlXTH23.5 and DlXTH25 during early SE by qRT−PCR. (D) Expression profiles of TFs (DlWRKY31, DlERF1, and DlERF5) during early SE by qRT−PCR. The data are expressed as the mean ± SD (n = 3). Different letters indicate significant differences. (E) Dual-luciferase assays of DlXTH-activating and candidate gene promoters in tobacco leaves. (F, G) Left: Image of firefly luciferase luminescence signals for the indicated reporter, and effectors were injected into tobacco leaves. Right: Relative reporter activity (LUC/REN) in tobacco leaves expressing the indicated reporters and effectors (Student’s t-test, **P < 0.01).