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. 2022 Nov 23;13:1043464. doi: 10.3389/fpls.2022.1043464

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Copyright © 2022 Ma, Chen, Liu, Xue, Zhang, Xu, Lai and Lin

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Transient expression of DlXTH23.5/25 in tobacco and functional verification of DlXTH23.5/25 with associated transcription factors (TFs). (A) Multiple sequence alignment of the DlXTH23.5/25 protein. (B) Subcellular localization of the DlXTH23.5–GFP and DlXTH25–GFP fusion proteins in tobacco leaves. Free GFP served as a control. CW, cell wall; CM, cell membrane. Scale bar = 20 μm. (C) Xyloglucan content during early somatic embryogenesis (SE) in longan. (D) Left: Xyloglucan content in wild-type and DlXTH23.5/25-overexpressing leaves. Right: Xyloglucan content in wild-type and DlWRKY31- and DlERF1/5-overexpressing leaves (Student’s t-test, *P < 0.05, **P < 0.01).